NanoSIMS 50L

Starting at the end of 2017, the Center for Biostructural Imaging of Neurodegeneration (BIN) can offer the services of a new high performance instrument, a nanoscale secondary ion mass spectroscope (NanoSIMS 50L) from the company CAMECA, to analyze the composition of a variety of samples. The NanoSIMS 50L is consists of a unique ion beam microprobe that enables SIMS analyses with very high lateral resolution.

The NanoSIMS methodology will be applied in the BIN for the correlative imaging of specific biological organelles, and will thus provide first insights into local protein and organelle turnover in a variety of cells and tissues.
The NanoSIMS 50L is available not only to scientists of the Göttingen University and of the University Medical Center Göttingen, but also to external users. An application must be submitted to the Device Manager, before any samples can be analyzed (see below for name and contact details).

The NanoSIMS 50L was purchased from a joint large-scale equipment grant from the Ministry for Science and Culture of Lower Saxony (NMWK) and the German Research Council (DFG).

Information for Users

The University Medical Center Goettingen (UMG) is the provider institution of the NanoSIMS equipment system, the latter being housed in the BIN in a laboratory especially provided for it. The NanoSIMS equipment system may only be operated by the NanoSIMS’ head scientific officer and those of his staff who have been trained to operate it.

The NanoSIMS 50L is usually booked weeks or months in advance. Please be aware that we cannot offer you a timeslot at short notice, but we will make every effort to realize your scheduling needs within the given constrictions. Please address your scheduling enquiries to one of the following persons:

Ten latest publications

• The maintenance of oocytes in the mammalian ovary involves extreme protein longevity.
  Harasimov K, Gorry RL, Welp LM, Penir SM, Horokhovskyi Y, Cheng S, Takaoka K, Stützer A, Frombach AS, Taylor Tavares AL, Raabe M, Haag S, Saha D, Grewe K, Schipper V, Rizzoli SO, Urlaub H, Liepe J, Schuh M.
  Nat Cell Biol. 2024 Jul;26(7):1124-1138. doi: 10.1038/s41556-024-01442-7. Epub 2024 Jun 20.
  PMID: 38902423 Free PMC article (PubMed)
• Morphological correlates of synaptic protein turnover in the mouse brain.
  Li F, Bahr JN, Bierth FA, Reshetniak S, Tetzlaff C, Fornasiero EF, Wichmann C, Rizzoli SO.
  Life Sci Alliance. 2024 Aug 12;7(11):e202402793. doi: 10.26508/lsa.202402793. Print 2024 Nov (PubMed)
• Erythropoietin restrains the inhibitory potential of interneurons in the mouse hippocampus.
  Curto Y, Carceller H, Klimczak P, Perez-Rando M, Wang Q, Grewe K, Kawaguchi R, Rizzoli S, Geschwind D, Nave KA, Teruel-Marti V, Singh M, Ehrenreich H, Nácher J.
  Mol Psychiatry. 2024 Oct;29(10):2979-2996. doi: 10.1038/s41380-024-02528-2. Epub 2024 Apr 15 (PubMed)
• Age-dependent structural reorganization of utricular ribbon synapses.
  Michanski S, Henneck T, Mukhopadhyay M, Steyer AM, Gonzalez PA, Grewe K, Ilgen P, Gültas M, Fornasiero EF, Jakobs S, Möbius W, Vogl C, Pangršič T, Rizzoli SO, Wichmann C.
  Front Cell Dev Biol. 2023 Aug 10;11:1178992. doi: 10.3389/fcell.2023.1178992. eCollection 2023 (PubMed)
• Tracking cell turnover in human brain using 15N-thymidine imaging mass spectrometry.
  Roeder SS, Bonnin EA, Wu TD, Guerquin-Kern JL, Jabari S, Brandner S, Eyüpoglu IY, Gollwitzer S, Hamer HM, Gerner ST, Doeppner TR, Rummel C, Englund E, Heimke-Brinck R, Borst T, Daniel C, Amann K, Schlötzer-Schrehardt U, Tonchev AB, Roessler K, Schwab S, Bergmann O, Rizzoli SO, Huttner HB.
  Front Neurosci. 2023 Oct 5;17:1274607. doi: 10.3389/fnins.2023.1274607. eCollection 2023 (PubMed)
• High-resolution analysis of bound Ca2+ in neurons and synapses.
  Bonnin EA, Golmohammadi A, Rehm R, Tetzlaff C, Rizzoli SO.
  Life Sci Alliance. 2023 Oct 13;7(1):e202302030. doi: 10.26508/lsa.202302030. Print 2024 Jan (PubMed)
• Mycorrhizal C/N ratio determines plant-derived carbon and nitrogen allocation to symbiosis.
  Pena R, Bluhm SL, Ammerschubert S, Agüi-Gonzalez P, Rizzoli SO, Scheu S, Polle A.
  Commun Biol. 2023 Dec 5;6(1):1230. doi: 10.1038/s42003-023-05591-7 (PubMed)
• An iodine-containing probe as a tool for molecular detection in secondary ion mass spectrometry.
  Kabatas Glowacki S, Agüi-Gonzalez P, Sograte-Idrissi S, Jähne S, Opazo F, Phan NTN, Rizzoli SO.
  Chem Commun (Camb). 2022 Jul 5;58(54):7558-7561. doi: 10.1039/d2cc02290g (PubMed)
• A Reliable Approach for Revealing Molecular Targets in Secondary Ion Mass Spectrometry.
  Li F, Fornasiero EF, Dankovich TM, Kluever V, Rizzoli SO.
  Int J Mol Sci. 2022 Apr 21;23(9):4615. doi: 10.3390/ijms23094615 (PubMed)
• White matter integrity in mice requires continuous myelin synthesis at the inner tongue.
  Meschkat M, Steyer AM, Weil MT, Kusch K, Jahn O, Piepkorn L, Agüi-Gonzalez P, Phan NTN, Ruhwedel T, Sadowski B, Rizzoli SO, Werner HB, Ehrenreich H, Nave KA, Möbius W.
  Nat Commun. 2022 Mar 4;13(1):1163. doi: 10.1038/s41467-022-28720-y (PubMed)